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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Every road leads to Rome: therapeutic effect and mechanism of the extracellular vesicles of human embryonic stem cell-derived immune and matrix regulatory cells administered to mouse models of pulmonary fibrosis through different routes

Fig. 5

Inhibition of pulmonary fibrosis by IMRC-EVs delivered via different routes in a mouse model of BLM-induced pulmonary fibrosis. a, b H&E staining (a) and Masson staining (b) micrographs of paraffin sections of mouse lung tissue in each group. The blue filaments represent collagen fibers, indicating interstitial fibrosis. c, d Representative photomicrographs of Col-I (c) and α-SMA (d) immunostained sections from each group. e Semi-quantitative assessment was performed on day 21 using Ashcroft scoring method. f The content of hydroxyproline (HYP) was determined in lung tissues, which is a marker of collagen deposition. g The lung coefficients of mice in each group. h Western blot for Col-I and α-SMA protein expression in lung tissue from each group. Quantification of the relative Collagen I protein expression levels (i) and α-SMA protein expression levels (j). k Representative microCT image 21 days after BLM instillation. l Quantitative CT imaging was used to measure the lung volume (n = 4–6). The data are expressed as the mean ± SD.*P < 0.05, **P < 0.01, ***P < 0.001, and ns indicates no difference

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Stem Cell Research & Therapy

ISSN: 1757-6512