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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels

Fig. 3

Load-induced regulation of ERK1/2, TGF-β and BMP activity and determination of SOX9 protein. Protein lysates from AC- and MSC-derived cartilage on day 21 and day 35 were subjected to Western blotting. A Phosphorylated ERK1/2 and total ERK1/2. B Densitometric analysis of pERK Western blot bands normalized to β-actin bands (hatched bar: control, black bar: compressed; *p ≤ 0.05, Mann– Whitney U test vs. control). C Phosphorylated SMAD1/5/9 and total SMAD1/5. D Phosphorylated SMAD2 and total SMAD2/3. E SOX9. F Densitometry of SOX9 Western blot bands normalized to β-actin bands (hatched bar: control, black bar: compressed; *p ≤ 0.05, Mann–Whitney U test vs. control). β-actin was used as loading control. Dashed lines indicate separate blots. Representative results from 3–4 donors are depicted

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