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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels

Fig. 5

Influence of the BMP pathway on load-induced alterations of ECM synthesis. A MSC-seeded osteochondral units pre-cultured for 21 days were treated with 100 ng/mL recombinant BMP6 starting 1 h before loading, during loading (or free-swelling) and during 24 h of radiolabel incorporation. Changes in 35S-sulfate and 3H-proline incorporation per DNA are depicted relative to the free-swelling control set to 1 (n = 3 donors). B AC-seeded osteochondral units pre-cultured for 21 days were treated with 500 nM of the BMP receptor (ALK1/2/3) inhibitor LDN-212854 (LDN21) or DMSO beginning 21 h before start of loading, during loading and during radiolabel incorporation. Alterations in 35S-sulfate incorporation per DNA are depicted relative to the free-swelling control set to 1 (n = 4 donors)

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Stem Cell Research & Therapy

ISSN: 1757-6512