Fig. 3
WW2 domain of YAP and PPQY motif of CDX2 are involved in YAP-CDX2 interaction. A Schematic illustration of the domain organization of YAP showing the various deletion constructs cloned and used for experimental purposes. The amino acid residue numbers are indicated at the top of each construct. CC, Coil–coil domain; TAD, transactivation domain; WW, tryptophan-rich domains. B Immunoprecipitation of CDX2 with either FLAG-tagged full-length YAP or various deletion constructs of YAP using lysates from TSCs transfected with either FLAG-tagged full-length YAP or various deletion constructs of YAP. Immunoprecipitation was done using anti-FLAG antibody followed by immunoblot with anti-CDX2 antibody. Immunoprecipitation using an isotype matched antibody was used as a negative control. IgG heavy chain was used as loading control. C Schematic illustration of the domain organization of CDX2 showing the region deleted for the experimental purpose. The amino acid residue numbers are indicated at the top of each construct. DBD, DNA-binding domain. D Either full-length CDX2 or ΔCDX2 was transfected in SH-SY5Y cells along with full-length YAP and maintained for 48 h. Extracts were immunoprecipitated using anti-FLAG antibody and coimmunoprecipitation of YAP was analyzed using anti-YAP antibody. Total cell lysates were used as input samples in B and D