Fig. 6

LATS1 influences COFILIN phosphorylation. A Western blot analysis of pLIMK2^Thr505, COFILIN, pCOFILIN^Ser3 and CHRONOPHIN in trophoblast cells ectopically overexpressing LATS1 as compared to empty vector transfected cells. B Densitometric analysis of the protein bands from A. Band intensities of COFILIN and CHRONOPHIN were normalized using GAPDH as the loading control, whereas the level of pLIMK2^Thr505 and pCOFILIN^Ser3 was normalized relative to the basal level of the protein. Error bars shows the SEM of biological triplicate runs. Statistical analysis was performed using Student’s unpaired t-test, *p < 0.05; **p < 0.005, ns, nonsignificant