Table 1 List of primers and restriction enzymes used for cloning of various full-length and deleted constructs
Clone identity | Fragment | Primer sequence (5′–3′) | Restriction enzyme |
|---|---|---|---|
Fl-Yap | – | AATAGGTACCAATGGAGCCCGCGCAACAG | KpnI |
AATAGGATCCCTATAACCACGTGAGAAAG | BamHI | ||
ΔW1 | F1 | AATAGGTACCAATGGAGCCCGCGCAACAG | KpnI |
AATAGCTAGCATCATCAGGGATCTCAAA | NheI | ||
F2 | AATAGCTAGCGCCATGCTTTCGCAACTG | NheI | |
AATAGGATCCCTATAACCACGTGAGAAAG | BamHI | ||
ΔW2 | F1 | AATAGGTACCAATGGAGCCCGCGCAACAG | KpnI |
AATTGCATGCTGAGGCAGAATTCATCAGCG | PaeI | ||
F2 | AATAGCATGCCTGGACCCAAGGCTGGAC | PaeI | |
AATAGGATCCCTATAACCACGTGAGAAAG | BamHI | ||
ΔW1W2 | F1 | AATAGGTACCAATGGAGCCCGCGCAACAG | KpnI |
AATAGCTAGCATCATCAGGGATCTCAAA | NheI | ||
F2 | AATAGCTAGCGCCATGCTTTCGCAACTG | NheI | |
AATTGCATGCTGAGGCAGAATTCATCAGCG | PaeI | ||
F3 | AATAGCATGCCTGGACCCAAGGCTGGAC | PaeI | |
AATAGGATCCCTATAACCACGTGAGAA AG | BamHI | ||
Fl-Cdx2 | – | AATAAAGCTTATGTACGTGAGCTACCT | HindIII |
AATTGGTACCTCACTGGGTGACAGTGG | KpnI | ||
ΔCdx2 | F1 | AATAAAGCTTATGTACGTGAGCTACCT | HindIII |
AATACTCGAGGTTCAGGCCGCCGGAGTG | XhoI | ||
F2 | AATACTCGAGCAGTCCCCAGGGCCATCC | XhoI | |
AATTGGTACCTCACTGGGTGACAGTGG | KpnI | ||
Fl-Lats1 | – | AATAGCGGCCGCAATGAAGAGGGGTGAAAAG | NotI |
ATATGGTACCTAAACATACACTAGATCTCGGT | KpnI |