Fig. 4

Ultrastructural features of mESCs differentiated in monolayers. A–H—representative transmission electron microscopy pictures of undifferentiated (A–D) and differentiated (E–H) mESCs. N—nucleus; M—mitochondria; ER—endoplasmic reticulum. I–L—quantitative scatter plot bar graphs showing cell area (I), perimeter (J), circularity index (K), and mitochondrial number (L). Data were analysed by unpaired t-test with Welch’s correction. Data presented as mean ± SEM. ****P < 0.0001 versus undifferentiated cells. n = 10 cells in each group