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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Stage-specific regulation of signalling pathways to differentiate pluripotent stem cells to cardiomyocytes with ventricular lineage

Fig. 5

Characterization of mESCs differentiated by EB. A Quantitative line graphs showing the percentage of contracting EBs treated with RA inhibitor (+ RAi) or control (−RAi). Data were analysed by two-way ANOVA and presented as Mean ± SEM. *P < 0.05 versus previous time point; #P < 0.05 versus +RAi group at corresponding days. B, C Representative Ca2+ traces of a single EB dissected from −RAi and +RAi groups. The EBs were treated with Fluo-4 and electrically stimulated to visualize spontaneous Ca2+ release as functional characterization of contracting CMs. D Quantitative scatter plot bar graphs of RT-PCR analysis showing the expression of RYR2 in the treatment groups. Data were analysed by unpaired t-test with Welch’s correction and presented as mean ± SEM. *P < 0.05 versus –Rai group. n = 4 independent repeats

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