Fig. 7

Ultrastructural features of mESCs differentiated by EB. A–J—representative transmission electron microscopy pictures of –Rai (A–E) and +RAi (E–H) cells. Yellow arrowhead—glycogen granules; pink arrowhead—collagen fibrils; N—nucleus; M—mitochondria; ER—endoplasmic reticulum. K–N—quantitative scatter plot bar graphs showing cell area (K), perimeter (L), circularity index (M) and mitochondrial number (N). Data were analysed by unpaired t-test with Welch’s correction and presented as mean ± SEM. **P < 0.01 and ****P < 0.0001 versus −RAi group. n = 10 cells in each group