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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Dppa3 facilitates self-renewal of embryonic stem cells by stabilization of pluripotent factors

Fig. 3

Full length of Dppa3 stabilizes Uhrf1. A Schematic representation of genomic overexpression of Dppa3 tagged with Flag. Four exons of Dppa3 are shown in the white box. There are three truncated versions of Dppa3; the first is a full length of Dppa3 (coding 1–150 amino acids) tagged with Flag; the second is a truncated Dppa3 (coding 1–60 amino acids) tagged with Flag; and the third is a truncated Dppa3 (coding 61–150 amino acids) tagged with Flag. Three cell lines were marked as Dppa31–150, Dppa31–60, and Dppa361–150. Dppa3 (white box), Flag (blue box). B Left: Western blot analysis of Uhrf1, Dppa3, and Flag protein levels in ES cells with overexpression of Dppa3. Molecular masses (kDa) on right. Right: quantification of relative Dppa3 and Flag protein levels normalized to Tubulin by the ImageJ software. The data are presented as mean ± SEM. (n ≥ 3; *p < 0.05). C Left: Western blot analysis of Uhrf1 and Flag protein levels in control group, Dppa31−150, Dppa31−60 and Dppa361−150 after MG132 treatment (20 mM). Right: Quantification of Uhrf1 protein level normalized to Tubulin by the ImageJ software. The data are presented as mean ± SEM. (n = 3; *p < 0.05). D Left: Western blot analysis of Uhrf1 and Flag protein levels in control group, Dppa31−150, Dppa31−60 and Dppa361−150 after CHX treatment (2 mM). Right: Quantification of Uhrf1 protein level normalized to Tubulin by the ImageJ software. The data are presented as mean ± SEM. (n = 3; *p < 0.05)

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