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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Dppa3 facilitates self-renewal of embryonic stem cells by stabilization of pluripotent factors

Fig. 4

Dppa3 prevents Nanog degradation in ES cells. A Left panel: The protein levels of Nanog in control and Dppa3 OE ES cells were analyzed at the indicated time points (0 h, 1 h, 2 h, 3 h) after CHX treatment (2 mM). Right panel: Quantification of Nanog protein levels normalized to Tubulin by ImageJ software. The data are presented as mean ± SEM. (n = 3; *p < 0.05, **p < 0.01). B Immunofluorescence staining assay represents the location of Dppa3 in ES cells. Scale bar = 30 μm. C IF assay the co-localization of Dppa3 and Nanog in ES cells. Scale bar = 30 μm. D, E. Immunoprecipitation analysis of the interaction between Nanog and Dppa3 in ES cells using anti-Nanog antibody (D) and anti-Dppa3 antibody (E), respectively

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