From: Stem cell therapy for vocal fold regeneration after scarring: a review of experimental approaches
Animals (n) | Surgery type | Time after surgery before IMP, days | Cell type/carrier | Dose | Duration | Use of IS | |
---|---|---|---|---|---|---|---|
Kanemaru et al. [49] | Rats (4) | Exterior access Injury with a 32G needle | 0 | Xenogeneic (murine) BM-MSC expressing GFP | (1–3) × 105 | 8 weeks | No |
Hertegard et al. [23] | Rabbits (10) | Endolaryngeal Limited unilateral resection of VF with microforceps and microscissors | 0 | Xenogeneic (human) BM-MSC | 0.8 × 105 cells in 0.1 mL | 4 weeks | Yes, Tacrolimus |
Lee et al. [45] | Dogs (10) | Endolaryngeal Bilateral resection of the posterior third of VF with an electrocoagulator | 4 | Autologous AD-MSC/atelocollagen | (1.0–3.0) × 106 cells in 0.2 mL | 8–24 weeks | No |
Johnson et al. [52] | Nude rats (12) | Endolaryngeal Bilateral injury of the middle third of VF with a 27G needle | 30 | Xenogeneic (murine) BM-MSC expressing GFP/HA-based sECM | ND | 4 weeks | No |
Xu et al. [46] | Rabbits (40) | Endolaryngeal Limited resection of the anterior and middle third of VF | 3–5 | Autologous AD-MSC/collagen or HA | ND | 15, 40 days and 3, 6, and 12 months | No |
Svensson et al. [37] | Rabbits (12) | Endolaryngeal Limited bilateral VF resection using microforceps | 63 | Xenogeneic (human) BM-MSC | (0.8–1.0) ˣ 105 cells in 0.1 mL | 10 weeks | Yes, Tacrolimus |
Ohno et al. [50] | Dogs (12) | Endolaryngeal Bilateral VF resection using microforceps and microscissors | 60 | Autologous AD-MSC/atelocollagen sponge | 1 × 106 cells in 5 × 3 × 3-mm | 6 months | No |
Kim et al. [60] | Rabbits (24) | Endolaryngeal Local uni/ bilateral VF resection using microinstruments | 0 | Xenogeneic (murine) BM-MSC expressing GFP | 1 × 105 cells in 50 μL | 3 months | No |
Kim et al. [38] | Rabbits (40) | Endolaryngeal Local uni/ bilateral VF resection using microinstruments | 0 | Xenogeneic (human) AD-MSC/Alginate-HA hydrogel | 1 × 106 cells in 50 μL | 3 months | No |
Peng et al. [27] | Dogs (14) | Endolaryngeal Bilateral resection of the middle third of VF using a laser | 0 | Allogeneic MSC from epiglottis mucosa/collagen | 2 × 106 cells in 0.2 ml | 2–8 weeks | No |
Choi et al. [25] | Rabbits (24) | Endolaryngeal Bilateral VF injury with an electrocoagulator | 0 | Xenogeneic BM-MSC/Small intestine submucosa | 2 × 107 cells in 50 μL | 8 weeks | No |
Hu et al. [39] | Dogs (17) | Endolaryngeal Limited resection of the anterior and middle thirds of VF | ND | Autologous AD-MSC, vocal fold fibroblasts, differentiated AD-MSC | (3.0–4.0) × 105 cells in 3–4 ml | 15 and 40 days and 3 and 6 months | No |
Hiwatashi et al. [40] | Rats (70) | Endolaryngeal Limited unilateral VF resection using microscissors | 60 | Allogeneic AD-MSC and BM-MSC | 5.0 × 105 cells in 50 μL | 1 and 3 months | No |
Svensson et al. [57] | Rabbits (16) | Endolaryngeal Limited unilateral VF resection using microforceps | 0 | Xenogeneic (human) ESC | 104 cells in 0.1 mL | 6 weeks, 3 months | Yes, Tacrolimus |
Valerie et al. [53] | Rabbits (74) | Endolaryngeal Limited bilateral resection of the anterior and middle thirds of VF using microforceps | 540 (18 months) | Autologous AD-MSC | 1.0 × 104 cells in 0.1 mL | 3 months | No |
De Bonnecaze et al. [41] | Rabbits (20) | Exterior (median thyroidotomy) Unilateral injury of left VF with an electrocoagulator | 0 | Autologous AD-MSC | 2 × 106 cells in 0.1 mL | 6 weeks | No |
Shiba et al. [47] | Rabbits (8) | Exterior (midline laryngofissure) Unilateral removal of the mucosa along the whole VF length | 0 | Autologous AD-MSC/fibrin gel | NA | 4 weeks | No |
Imaizumi et al. [26] | Rats (30) | Endolaryngeal Unilateral VF injury with a 25G needle | 0 | Xenogeneic human iPSC/HA hydrogel | 1.0 × 105 cells in 5 μL | 1 and 2 weeks | No |
Morisaki et al. [61] | Rats (72) | Endolaryngeal Unilateral VF injury with a 25G needle | 0 | Allogeneic AD-MSC | 5.0 × 105 cells in 50 μL | 3, 14, 56 days | No |
Bartlett et al. [54] | Rabbits (84) | Endolaryngeal Bilateral resection of the middle VF third using microforceps | 42 | Xenogeneic (human) BM-MSC/HyStem-VF hydrogel | 3 × 105 cells in 100 μL twice or 1.5 × 105 cells in 100 μL | 2, 4, and 10 weeks | No |
Goel et al. [48] | Rabbits (8) | Exterior (midline laryngofissure) Unilateral removal of the mucosa along the whole VF length | 0 | Allogeneic AD-MSC/fibrin gel | NA | 4 weeks | No |
Svistushkin et al. [62] | Rabbits (12) | Endolaryngeal Limited unilateral VF resection using microforceps | 84 | Autologous BM-MSC | 6 × 105 cells in 0.6 mL | 12 weeks | No |
Hertegård et al. [22] | Rabbits (18) | Endolaryngeal Limited VF resection using microforceps | 0 | Xenogeneic (human) BM-MSC/HA hydrogel | 1 × 105 cells in 0.1 mL | 3 and 25 days | No |