From: Stem cell therapy for vocal fold regeneration after scarring: a review of experimental approaches
In vitro characterization | Cell type and detection method after IMP | Markers for MA | Histological staining | Markers for IHC | Morphometric parameters | In vivo assessment | Physical properties | ||
---|---|---|---|---|---|---|---|---|---|
Mechanics | Vibration | ||||||||
Kanemaru et al. [49] | FACS (Sca-l, CD29, CD34, CD44, CD45, CD4ge, and 7-AAD) | Xeno (murine) BM-MSC GFP tracing | No | H&E | Keratin Desmin | No | No | No | No |
Hertegard, et al. [23] | FACS (CD73, CD90, CD105, CD166, CD14, CD31, CD34, CD45, and CD80) | Xeno (human) BM-MSC FISH | No | H&E | Collagen type I | Collagen density | No | No | No |
Lee et al. [45] | No | Auto AD-MSC CM-Dil tracking | No | H&E | No | No | Direct laryngoscopy | No | No |
Johnson et al. 2010 [52] | – FACS (CD11b, CD19, CD-45, 7-AAD) – AlamarBlue assay – ICC (CD-44, Sca-1) | Xeno (murine) BM-MSC GFP tracing | COL3A1 FN1 HAS3 HYAL3 SMA TGF- β1 | No | No | No | No | No | No |
Xu et al. [46] | – Phase contrast microscopy – MTT assay – FACS (CD34, CD44, CD105, CD106) – ICC (Vimentin) – HS (Oil red O, Von Kossa) – SEM | Auto AD-MSC DAPI tracking | No | H&E MT AB | FN | Collagen, HA, FN density | Endoscopy | No | No |
Ohno et al. [50] | No | Auto BM-MSC ND | No | vG AB | No | LP thickness Collagen and HA density | No | No | No |
Kim et al. [60] | No | Xeno (murine) BM-MSC GFP tracing | COL1A1 HAS2 FN1 TGF- β1 | H&E MT AB | Collagen type I | Collagen and GAG density | Endoscopy | No | No |
Kim et al. [38] | – FACS (CD73, CD90, CD31, CD34, CD-45) – Differentiation test (AG, OG, CG) | Xeno (human) AD-MSC CM-DiI tracking | No | H&E MT VvG | HGF FSP-1 Collagen type I FN | Collagen, elastin, and FN density | Endoscopy | No | No |
Peng et al. [27] | – FACS (CD29, CD44, CD90, CD105e, CD34, CD45) – Clonogenicity assay – Differentiation test (AG, OG, CG) | Allo EM-MSC Dil tracking IHC (SMA, vimentin) | No | H&E MT vG AB | FN | Relative content of collagen, elastin and HA | Comparativeendoscopy | No | No |
Choi et al. [25] | No | Xeno BM-MSC In vivo DiD tracking | – | H&E MT AB PR | Scar index measurement Intensity of collagen optical density | Endoscopy | No | No | |
Hu et al. [39] | – Phase contrast microscopy – ICC (vimentin, collagen) | Auto AD-MSC/VF fibroblasts/dAD-MSC ND | No | No | Collagen Elastin HA Decorin FN | No | Endoscopy | No | No |
Hiwatashi et al. [40] | No | Allo (murine) BM-MSC and AD-MSC GFP tracing | FGF2 HGF COL1A1 COL3A1 HAS1 HAS2 HAS2 MMP1 VEGFa | vG AB | No | LP thickness collagen and HA density | No | No | No |
Svensson et al. [57] | No | Xeno (human) ESC FISH | No | H&E | Collagen type I | LP thickness Collagen density Scored fibrosis analysis | No | No | No |
Valerie et al. [53] | No | Allo AD-MSC ND | No | H&E MT AB RO | No | LP thickness Scored collagen, elastin and HA density | No | No | No |
De Bonnecaze et al. [41] | – FACS (CD34, CD45, CD31, CD73, CD90) – Differentiation test (AG, OG) | Auto AD-MSC GFP tracing via LV transduction | No | H&E PR O | Collagen type I FN | LP and EP thickness PR optical density Density of elastic fibers Number of inflammation foci (foci/mm3) | No | No | No |
Shiba et al. [47] | – HA (H&E, vG, MT) – IHC (vimentin, panCK) | Auto AD-MSC in fibrin gel ND | No | H&E vG MT | No | No | Endoscopy | No | No |
Imaizumi, et al. [26] | No | Xeno (human) iPSC FISH | No | H&E vG MT | panCK AFP ALPP | Collagen and elastin density Retention of hydrogel | No | No | No |
Morisaki et al. [61] | FACS (CD29, CD90, CD45) | Allo AD-MSC GFP tracing | HAS1 HAS2 HAS3 COL1A1 COL3A1 MMP1 MMP8 FGF2 HGF VEGFa | vG AB | No | Collagen and HA density | No | No | No |
Bartlett et al. [54] | No | Xeno (human) BM-MSC Human β-actin expression | COL1A2 COL3A1 FN1 FMOD LPL OCN HAS HYAL2 IL1β IL17β, TGF-β1, TNF INγ αSMA | MT VvG AB | No | Relative collagen, elastin and HA content | No | No | No |
Goel et al. [48] | No | Allo AD-MSC FISH, PCR (Y CS) TUNEL labeling for apoptotic cells | No | H&E MT | No | No | No | No | No |
Svistushkin et al. [62] | No | Auto BM-MSC ND | No | H&E PR O | Collagen type I and III | LP thickness Thickness of collagen fibrils Scored analysis of 11 morphological parameters Collagen type I/III ratio | No | No | No |
Hertegård et al. [22] | – FACS (CD73, CD90, CD105, HLA-I, CD14, CD34, CD45, HLA-II) – Migration and invasion assay – qPCR (HAS, HYAL, MMP) – ELISA (IL8, HGF, IL6, VEGF, TGF-β1) | Xeno (human) MSC FISH | No | AB | Collagen type I | Fibrosis, inflammation, MPS intensity Scored fibrosis analysis | No | No | No |