Fig. 5

Inhibition of ErbB receptor suppressed ERK signaling. A WB detection for p-ERK1/2, p-AKT and Survivin in A549 cells and H1299 treated with cisplatin or taxol for 24 h. B WB detection for p-ERK1/2 and Survivin in A549-TR cells and H1299 cells treated with afatinib for 12 h. C, D The cell viability of A549 -TR cell (C) or H1299 cells (D) treated with 100 ng/mL taxol plus with 1 µM Selumetinib for 48 h, n = 3. E, F The representative images (E) and the statistics data (F) of A549-TR spheres treated with 100 ng/mL taxol plus with 1 µM Selumetinib, n = 3. G, H The representative images (G) and the statistics data (H) of H1299 spheres treated with 100 ng/mL taxol plus with 1 µM Selumetinib, n = 3. Scale bars, 100 μm in black; TR, taxol resistance; n.s., no significance; *, p < 0.05; **, p < 0.01