Fig. 6

Downregulation of EREG re-sensitized NSCLC to chemo-drugs through ERK signaling. A WB detection for p-ERK1/2, Survivin and EREG in A549 and H12999 cells reconstituted with shEREG. B The cell viability of shEREG A549-TR cells treated with 100 ng/mL taxol for 48 h, n = 3 (C, D) The sphere forming of shEREG A549-TR (C) or shEREGH1299 cells (D) treated with 100 ng/mL taxol, n = 3. E, F The cell viability of shEREG-A549 cells (E) or shEREG-H1299 (F) treated with serial taxol for 48 h, n = 3. G The RT-qPCR detection of stemness-associated genes in A549-TR cells with shEREG, n = 3. H The HRs of positive resistant genes between highly expressed population and lowly expressed population in variant cancer. I Proposed schematic mechanism of EREG regulating stem-like properties in NSCLC via ERK pathway. HR, hazard ratio; CIs, confidence interval; TR, taxol resistance; n.s., no significance; *p < 0.05; **p < 0.01