Fig. 5

MEECs transplantation and repair intrauterine injury. A Trichrome-staining of murine uterine tissues. Uterine horns were collected from sham operated/control group, non-transplanted/injury group, and cells-transplanted group at three estrous cycles (day 14) after uterus damage/cell transplantation. Tissues were fixed by 4% paraformaldehyde (w/v), paraffin-embedded, and sectioned using standard histological procedures. The result of staining was photographed under the microscope. Scale bar, 50 μm. Blue staining indicated the fibrosis. B P63 expression in murine endometrium tissue at day 21. Sectioned tissues were detected by DAB staining. Scale bar, 100 μm. C Comparison of murine endometrial structure by trichrome-staining. Uterine horns were collected from sham operated/control group, non-transplanted/injury group, and cells-transplanted group at day 21, 30, and 45 after uterus damage/cell transplantation. Tissues were fixed, paraffin-embedded, and sectioned using standard histological procedures. The representative results of staining were shown. Scale bar, 50 μm. D Comparison of area of fibrosis, gland numbers, and thickness of endometrium. The gland numbers, thickness of endometrium, and area of fibrosis were counted or measured in relatively set views. Data were presented as mean ± SD (n = 3). **p < 0.01, ***p < 0.001