Fig. 1
From: Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model
Regeneration and characterization of the in vitro cultured mouse lens by the capsular bag culture model. A Pattern of the establishment of an in vitro mouse lens capsular bag culture model. B The diagram on the left column shows the pattern of the lens regeneration; the progression of regeneration of the cultured lenses was followed by microscopy and by staining of β-crystallin (green) at days 1, 7, and 14. Nuclei were stained with DAPI (blue). C The mRNA levels of the LEC-related and LFC-related genes were assessed by qPCR 14 days after in vitro culture and compared with the levels at day 1 (n = 4). Data are presented as mean ± SD. Unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001