Fig. 2
From: Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model
Effect of NAM on the transparency and differentiation of the regenerated lens after 28 days of follow-up. A General images of the regenerated lenses with or without NAM treatment and H&E staining. B Light transmittance of the two lens groups at different wavelengths. Data are means ± SD of 4 regenerative lenses per group. C Representative immunofluorescence staining showing the expression of the LEC markers PAX6 (green), αA-crystallin (green), and αB-crystallin (green), and the LFC markers PROX1 (green), β-crystallin (green), and MIP (red) in the NAM-treated (NAM) and untreated control (CON) lens groups. A normal lens (NOR) was used as a control. Nuclei were stained with DAPI (blue). D The qPCR results evaluating the genes of LECs and LFCs in the untreated control (CON) and NAM-treated groups (n = 4). Data are presented as mean ± SD. Unpaired t-test. *p < 0.05, **p < 0.01