Fig. 2
From: Meniscus repair: up-to-date advances in stem cell-based therapy
Copyright 2018, Jayasuriya et al.
Establishment of healthy human articular cartilage-derived progenitor cell lines [91]. Illustrated diagram of procedure used to establish human cartilage progenitor cell lines. Healthy human cartilage was diced into small pieces and digested using pronase and collagenase. Released cells were washed and strained to remove clumps. Cells were seeded at low density and individual single-cell-derived colonies were separated and stabilized using SV-40 mediated delivery of large-T antigen. Reproduced under the terms of the CC BY-NC license [91].