Fig. 2

Senescence markers are downregulated in AT2 cells of MSCs-treated pulmonary fibrosis mice. A Immunofluorescence staining of lung sections from mice (n = 6 per group) and visualized using anti-P16 (green) and anti-SPC (red) antibodies. Scale bars: 50 μm. B SA-β-galactosidase staining of primary AT2 cells from mice of the different groups (n = 6 mice per group). C Quantification of the percentage of β-galactosidase positive cells from B. D qPCR analysis of P16 mRNA expression in primary AT2 cells from mice of the different groups. E qPCR analysis of P21 mRNA expression in primary AT2 cells from mice of the different groups. F Western blot analysis of P16 and P21 expression in primary AT2 cells from mice of the different groups. G qPCR analysis of IL6 mRNA expression in primary AT2 cells from mice of the different groups. H qPCR analysis of IL8 mRNA expression in primary AT2 cells from mice of the different groups. Data are presented as the mean ± SEM of three independent experiments; *P < 0.05, ***P < 0.001; one-way ANOVA and Tukey’s multiple comparisons test