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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: The role of serum amyloid A1 in the adipogenic differentiation of human adipose-derived stem cells basing on single-cell RNA sequencing analysis

Fig. 7

SAA1 changed during the adipogenic differentiation of human ASCs. A Protein expression analysis of SAA1 and adipogenic differentiation protein markers after adipogenic-induced one week. GAPDH was used as the internal reference gene. Mean ± SD, *p < 0.05, n = 3. GM: human ASCs were cultured in growth medium; AD1W: human ASCs were cultured in adipogenic inducing medium 1 week. B Morphological changes of SAA1 during the adipogenic differentiation of ASCs. SAA1 is marked in red, microfilaments are marked with phalloidin in green, and the nucleus is marked with DAPI in blue. Scale bar = 50 µm. Mean ± SD, ** P < 0.01, n = 3. C Oil red O staining for triglyceride content and quantitative analysis in SAA1-silenced ASCs (siSAA1) after 4 days of adipogenic differentiation. Negative control group was siControl group. Scale bar = 100 μm. Mean ± SD, ****p < 0.0001, n = 3

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