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Fig. 2. | Stem Cell Research & Therapy

Fig. 2.

From: Development and evaluation of a novel xeno-free culture medium for human-induced pluripotent stem cells

Fig. 2.

253G1 hiPSCs on iMatrix-511 and GNF with ON2 medium maintained normal karyotype and differentiation ability. a Normal karyotypes of 253G1 hiPSCs maintained on both iMatrix-511 and GNF over 20 passages with ON2 were identified by G-band karyotype analysis (46 XX). b All three germ layer differentiation in vitro of 253G1 hiPSCs occurred after expansion on iMatrix-511 or GNF with ON2. Ectodermal, mesodermal, and endodermal cell types were revealed by specific markers: β-tubulin III, myosin, and AFP, respectively, by immunocytochemistry. Scale bar, 100 µm. c Histological analysis of teratomas generated in SCID mice from 253G1 iPSCs after ON2 culture on iMatrix-511 or GNF, respectively. Neuro-epithelium, cartilage, and gut-like epithelium were visualized by hematoxylin and eosin staining. Scale bar, 200 µm

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