Fig. 2
From: Large-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
Upscaling of nephron sheets to a diameter of at least 4 cm. A Image of large-scale nephron sheet of 12.6 cm2 made with a 4 cm diameter cover template (iPSC-MAFB). B, C Immunofluorescence analysis of the entire nephron sheet shows presence and equal distribution of kidney tubular structures stained for proximal (LTL, B) and distal (ECAD, C) markers. D Section of the large nephron sheet highlights the distribution of tubular structures stained for LTL and ECAD combined. E Detection of nephron structures after immunofluorescence staining for glomerular structures (NPHS1), proximal tubules (LTL), and distal tubules (ECAD)