Fig. 3

hiPSC-derived nephron sheets reproducibly contain kidney structures and show high number of glomerular structures. A Immunofluorescence analysis for glomerular structures (NPHS1, NPHS2), endothelium (CD31), proximal tubule (LTL, CUBN), distal tubular and collecting duct structures (ECAD) in whole mount nephron sheets, and stromal cells (MEIS1/2/3, PDGFRα/β) in cryosections using LUMC0072, LUMC0020 and iPSC-MAFB (representative images from 3 independent experiments). B Immunofluorescent 3-dimensional overview showing distribution of NPHS1, CD31 and LTL in bisected hiPSC-derived nephron sheet. C Diameter of hiPSC-derived nephron sheets using an 18 mm template in multiple hiPSC lines. (LUMC0072: 32 nephron sheets in 11 independent experiments, LUMC0020: 5 nephron sheets in 3 independent experiments, iPSC-MAFB: 11 nephron sheets in 6 independent experiments). D Volume of individual glomerular structures (µm³) of organoids and nephron sheets. Each dot represents the volume of a single glomerular structure determined in LUMC0072: 7 organoids from 4 independent experiments and 3 nephron sheets from 3 independent experiments; LUMC0020: 6 organoids in 6 independent experiments and 3 nephron sheets in 3 independent experiments; iPSC-MAFB: 4 organoids in 4 independent experiments and 3 nephron sheets in 3 independent experiments. Bar displays average. E Number of glomerular structures in organoids and nephron sheets. Total glomerular number was determined in LUMC0072: 4 organoids from 4 independent experiments and 4 nephron sheets from 4 independent experiments; LUMC0020: 5 organoids in 5 independent experiments and 3 nephron sheets in 3 independent experiments; iPSC-MAFB: 3 organoids in 3 independent experiments and 3 nephron sheets in 3 independent experiments. Bar displays average