Fig. 5
From: Large-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
Cryopreservation of hiPSC-derived nephron sheets. A Schematic of cryopreservation procedure. hiPSCs are differentiated until day 7 of differentiation, dissociated to single cells and cryopreserved. Cells are thawed and differentiation can be continued using both templates for hiPSC-derived nephron sheets. B Brightfield images of nephron sheets without (control) and with cryopreservation (representative image of 4 independent experiments). C Immunofluorescence analysis of glomerular structures (NPHS1, NPHS2), endothelium (CD31), proximal tubules (LTL, CUBN), distal tubular structures (ECAD) in whole mount control and cryopreserved nephron sheets (4 independent experiments)