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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human integrin α10β1-selected mesenchymal stem cells home to cartilage defects in the rabbit knee and assume a chondrocyte-like phenotype

Fig. 4

Trilineage differentiation of unlabeled (UL-MSCs) and 6h-MIRB-labeled (6-h MIRB-MSCs) integrin α10-MSCs. A Chondrogenic differentiation was induced and evaluated by qPCR analysis of collagen type II (COL2), aggrecan and integrin α10 in UL-MSCs or 6h-MIRB-MSCs. Uninduced UL-MSCs and 6h-MIRB-MSCs served as controls. The relative quantity of all genes was normalized to chondrogenically induced UL-MSCs. Uninduced integrin α10-MSCs (both UL-MSCs and 6h-MIRB-MSCs) showed no expression of COL2 or aggrecan but did show expression of integrin α10. IntgA10 = integrin α10; MIRB = Molday Ion conjugated with Rhodamine B. B Adipogenic and osteogenic differentiation was confirmed by positive staining of intracellular lipid droplets with Oil Red O and extracellular calcium deposits with Alizarin Red, respectively. There was no visual difference between UL-MSCs or 6h-MIRB-MSCs

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Stem Cell Research & Therapy

ISSN: 1757-6512