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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Regeneration difficulties in patients with FQAD can limit the use of iPSc-based cell therapy

Fig. 2

Characterization of the iPS cells from donor 9. a Representative images showing iPSc generated from donor 9 urinary cells immunostained with pluripotency markers SOX2 (green) and OCT3/4 (red), TRA-1-60 (red), TRA-1-81 (red) and morphology of the cell culture under self-renewal conditions. b Karyotype analysis indicated karyotypically normal iPS cells. c Representative images showing iPSc differentiation into three germ layers: MAP2 (green)—ectoderm, SOX17 (green)—endoderm, αSMA (red)—mesoderm. Each image was taken at magnification 200×. For better readability, the light microscopy images were contrast-and brightness-enhanced. Scale bars represent 100 μm and 500 μm (immunofluorescence and bright field images, respectively)

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