Fig. 3

Sorafenib inhibition of HHUA cell properties and stem cell characteristics. A Reduction of HHUA-SP and -MP cell numbers by treatment with sorafenib (soraf), as determined using an automated cell counter. Each dot indicates the mean ± SEM obtained from three independent experiments using three individual samples. *, P < 0.05 vs. DMSO control (ctrl), based on Student’s t-test. B The effects of soraf and itraconazole (itrac) on HHUA-SP and -MP cell proliferation, as determined by the MTS assay. Each point indicates the mean ± SEM absorbance at 490 nm obtained from three independent experiments using three individual samples. *, P < 0.01, †, P < 0.02 versus each control, based on Student’s t-test. C Inhibition of HHUA-SP and -MP cell invasion by soraf. Each bar indicates the mean ± SEM number of invading cells obtained from three independent experiments using three individual samples. Images (insert) show representative Giemsa-stained membranes followed by treatment as indicated. *, P < 0.05 vs. DMSO control, based on Student’s t-test