Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Dextran sulfate prevents excess aggregation of human pluripotent stem cells in 3D culture by inhibiting ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway

Fig. 2

The effect of DS on the expression of CAMs on the H9 aggregates in 3D suspension culture conditions. A–D Gene expression analysis by qRT-PCR for CAMs-related genes (A), cell adhesion-associated genes, inhibitors of the matrix metalloproteinases, and TGFβi (B), pluripotent genes (C), and E-cad and ICAM1 (D) after treatment with various DS concentrations. Relative gene expression represents data normalized to GADPH and expressed relative to untreated H9 aggregates. Data represent the mean ± SD. *P < 0.05 and **P < 0.01. Abbreviations: OCT4, POU class 5 homeobox 1; NANOG, Nanog homeobox; SOX2, SRY-box transcription factor 2; P-cad, cadherin 3; TIMP, TIMP metallopeptidase inhibitor; ITGA, integrin subunit alpha; TGFβi, transforming growth factor beta induced; VTN, vitronectin; ECM1, extracellular matrix protein 1; PECAM, platelet and endothelial cell adhesion molecule 1; E-cad, cadherin 1; ICAM1, intercellular adhesion molecule 1

Back to article page