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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Dextran sulfate prevents excess aggregation of human pluripotent stem cells in 3D culture by inhibiting ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway

Fig. 3

The expression of ICAM1 was significantly different between naturally developed big and small aggregates of the H9 cells. A Representative images of big and small aggregates of the H9 cells on day 5 in 3D suspension culture condition without any extra treatment; these two groups were separated by natural settlement. Scale bar = 200 μm. B Comparison of average diameter of big and small aggregates of the H9 cells on day 5. C Diameter distribution of big and small aggregates of the H9 cells on day 5. D–F Gene expression analysis by qRT-PCR for pluripotent genes (D), CAMs-related genes (E), and ICAM1 (F), relative gene expression represents data normalized to GADPH and expressed relative to untreated H9 aggregates. G The protein levels of E-cad and ICAM1 were determined by western blotting in big and small aggregates of the H9 cells. H, I The densitometry for the protein levels of E-cad (H) and ICAM1 (I) were quantitated in G; GAPDH was used as a loading control. Data represent the mean ± SD. *P < 0.05 and **P < 0.01 and ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512