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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Dextran sulfate prevents excess aggregation of human pluripotent stem cells in 3D culture by inhibiting ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway

Fig. 7

The effect of DS on adhesion of the H9 cells could be partially rescued by Wnt inhibitor. A AP staining of the H9 colonies treated with or without DS, supplemented with 5 μM Wnt inhibitor IWR-1 or IWP2, single cells were seeded in six-well culture plates coated with Matrigel, 10 μM Y-27632 was added on day 1 in all groups. Representative images were shown. Scale bar = 200 μm. B, C Quantification of the efficiency of AP+ colonies (B) and the average colony area (C) in A. D Schematic diagram showing DS prevents the adhesion of the aggregates of the H9 cells through modulating the expression of ICAM1 and the canonical Wnt signaling pathway. Treating the aggregates with DS leads to the down-regulation of the expression of ICAM1. The expression of secreted Wnt ligands and receptor such as Wnt7B and FZD8 is up-regulated in 3D suspension culture conditions after DS treatment, causing the activation of the canonical Wnt signaling pathway, which impedes the degradation of cytosolic β-catenin and promotes its transference into the nucleus, thus further up-regulating the expression of Wnt target genes, consequently down-regulating the transference expression of E-cad. Data represent the mean ± SD. *P < 0.05 and **P < 0.01 and ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512