Fig. 2

Musashi-1 knockdown leads to decreased cell proliferation and metabolism in vitro. A Plating efficiency of Musashi-1 depleted cells (using two siRNAs for the knockdown) compared to control cells in Ishikawa (A1, p < 0.001, n = 14) and KLE cell line (A2, p = 0.027, n = 20). B Representative images. C Decreased colony formation after Musashi-1 knockdown (Msi-1 KD) is partly restored after additional knockdown of p21 (Msi-1/p21 KD) in siPOOL experiments. (C1 Ishikawa: mean plating efficiency 0.189 for ctrl, 0.146 for Msi-1 KD and 0.167 for Msi-1/p21 KD, p = 0.003 for Msi-1 KD compared to controls, p = 0.041 for Msi-1 KD compared to Msi-1/p21 KD and p = 0.076 for Msi-1/p21 KD compared to controls; C2 KLE: mean plating efficiency 0.014 for ctrl, 0.008 for Msi-1 KD and 0.011 for Msi-1/p21 KD, p = 0.003 for Msi-1 KD compared to controls, p = 0.036 for Msi-1 KD compared to Msi-1/p21 KD and p = 0.063 for Msi-1/p21 KD compared to controls). 2D: Metabolic activity of Musashi-1 knockdown cells compared to control cells, measured by MTT assay (D1 Ishikawa p = 0.003, n = 5; D2 KLE p = 0.002, n = 4)