Fig. 3

Musashi-1 downregulation is associated with changes in cell cycle, cancer stem cell and proliferation genes. A TERT (telomerase reverse transcriptase) expression is downregulated on both, mRNA (A1, Ishikawa p = 0.003 (mean delta ct values 20.27 for ctrl, 21.12 for Msi-1 depleted cells); KLE p = 0.018 (mean delta ct values 21.35 for ctrl, 22.49 for Msi-1 depleted cells); both n = 4) and protein (A2, Ishikawa p = 0.014 (relative protein expression level normalized to tubulin 0.23 for ctrl and 0.13 for Msi-1 KD), KLE p = 0.001 (relative protein expression level normalized to tubulin 5.71 for ctrl and 2.20 for Msi-1 KD); both n = 4) level in both cell lines. B Increased numb (p = 0.047, n = 4, relative protein expression level normalized to tubulin 4.50 for ctrl and 6.18 for Msi-1 KD) and decreased Notch-1 protein expression (p = 0.049, n = 3, relative protein expression level normalized to tubulin 0.14 for ctrl and 0.10 for Msi-1 KD) in the KLE cell line after Msi-1 knockdown. C Numb protein expression in Ishikawa cells is increased after Msi-1 silencing (p = 0.044, n = 4, relative protein expression level normalized to tubulin 1.87 for ctrl and 3.26 for Msi-1 KD). D Expression of p21 in KLE after Msi-1 knockdown (p = 0.048, n = 4, relative protein expression level normalized to tubulin 2.36 for ctrl and 3.98 for Msi-1 KD). Representative Western Blot signals are shown in Additional file 1: Fig. S4