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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing

Fig. 2

Green OLED irradiation-induced cellular changes in hADSCs. Gene expression levels of OPN3, and OPN4 in hADSCs at 0, 24, and 48 h after green OLED irradiation for 4 h (a) or 24 h (b) as evaluated by qRT-PCR (n = 5). c Western Blot analysis of OPN3 and OPN4 expression in hADSCs at 0 and 24 h after green OLED irradiation for 24 h with quantification results (n = 3). d Gene expression levels of PLC-β, and IP3R in hADSCs at 0 and 24 h after green OLED irradiation for 24 h as evaluated by qRT-PCR (n = 5). e Quantification of Ca2+ in hADSCs after irradiation for 24 h as evaluated by calcium assay (n = 4). f Quantification of ROS in hADSCs after irradiation for 24 h as evaluated by cellular ROS assay (n = 6). g Representative H2DCFDA staining images of hADSCs after green OLED irradiation for 24 h (scale bar = 250 µm). h Quantification of ATP in hADSCs after green OLED irradiation for 24 h as evaluated by ATP assay (n = 4). i Schematic of OPN4 molecular mechanism. *p < 0.05; **p < 0.001 compared to no treatment group with unpaired t test

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