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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic inflammatory microenvironment

Fig. 5

SNHG8 directly binds to HIF-1α. A The catRAPID heat-map shows the prediction of the interaction between SNHG8 and HIF-1α. The x- and the y-axes represent the indexes of the RNA and protein sequences, respectively. The colors of the heat-map indicate the interaction score (ranging from − 3 to + 3) of the individual amino acid and nucleotide pairs. The interaction is identified with confidence (interaction propensity = 120; discriminative power = 99%). B The interaction is characterized by a binding peak. The x- and the y-axes represent the indexes of the RNA sequence and the interaction score, respectively. C qRT-PCR shows the RNA enrichment in the RIP assay using the anti-HIF-1α antibody in PDLCs. IgG was used in the control group as the nonspecific protein. D Western blot analyses show the protein enrichment in the RNA pulldown assay. Histograms show the quantification of band intensities. (Analysis of variance **p < 0.01; ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512