Fig. 6

Expression of Bmp6, Fgf18, and Wnt signaling molecules is disturbed in hairpoor mouse. a Immunohistochemical staining of Bmp6 (green) and Fgf18 (red) in telogen phage. The arrow indicates Bmp6 and Fgf18 doubly positive cells and the arrowhead Bmp6 only positive cells. Nuclei were counterstained with DAPI. Bulge. Scale bar = 50 μm. b, c Expression level of Bmp6 and Fgf18 of the epidermis at the 1st and 2nd telogen phase of the hairpoor and the wild-type mouse determined by qRT-PCR analyses. d,e,f,g Expression level of the Wnt signaling molecules (Sfrp1, Axin2, Wnt7b, Wnt10b) in the stage of telogen total skin of the hairpoor and wild-type mouse determined by qRT-PCR analyses. h Schematic of the telogen status of each mouse. Expression level was normalized against that of Gapdh, and the relative expression level was determined using those of the wild-type levels as reference. n ≥ 3 per genotype. Data are mean ± SD. *P < 0.05, ***P < 0.001