Fig. 2
From: Assay establishment and validation of a high-throughput organoid-based drug screening platform
Explorations on the appropriate step size of Z-stack. A The size distribution of surviving tumor organoids on the tenth day after chemotherapy treatment, as determined by short diameter measurement. B The size distribution of regenerated intestinal organoids on the fifth day post 8 Gy irradiation, as determined by short diameter measurement. C Examples of imaging of microspheres of different sizes when using different numbers of stacks to screen. The range of performing Z-sack was 1100 μm. D, E The numbers and areas of microspheres varied with the different numbers of Z-stack layers. *Organoids that were blurred became clear with the increase of stacks