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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Assay establishment and validation of a high-throughput organoid-based drug screening platform

Fig. 4

Fluorescence dye could effectively identify living or dead cells in organoids. A Representative image of an illegible tumor organoid for researchers to identify under bright fields. After being treated with 5-FU, the organoid was composed of loose peripheral dead cells and an area with unsure living or dead cells. B Comparison of the staining effects of different fluorescent dyes. C Calcein-AM and PI can effectively mark living or dead cells in patient-derived tumor organoids before and after treatment. D Calcein-AM and PI identified living or dead cells in mouse small intestinal organoids. Neutral red staining was performed at the same time, and the results were consistent with Calcein-AM staining. E Comparison of the cross-sectional areas of organoids under bright field and fluorescent field on the fifth day after 8 Gy irradiation. Data are presented as the mean \(\pm\) SEM, n = 39. F Comparison of the time required to delineate the areas of the surviving organoids under the bright field and fluorescent field, n = 5. Data are presented as the mean \(\pm\) SD, n = 5. *P < 0.05, ***P < 0.001

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