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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human amniotic mesenchymal stem cells-derived IGFBP-3, DKK-3, and DKK-1 attenuate liver fibrosis through inhibiting hepatic stellate cell activation by blocking Wnt/β-catenin signaling pathway in mice

Fig. 4

Effects of hAMSCs and hAMSC-CM on activation, apoptosis, and proliferation in LX-2 cells. A The expression levels of α-SMA and TGF-β in LX-2 cells were determined by immunofluorescence staining. B Schematic diagram of the control group, hAMSC-CM group, and co-culture group. C LX-2 cells were treated with normal medium (control), hAMSCs, and hAMSC-CM. The expression levels of Collagen I, Collagen III, TGF-β, α-SMA, PCNA, and Bcl-2 in LX-2 cells of different groups were analyzed by western blot after 48 h of treatment. D The apoptosis of cells was assessed by FACS. E Quantitative analysis of the percentage of apoptotic cells and survival cells as shown in (D). F Representative images of LX-2 cells observed under a light microscope after cultured with normal medium, hAMSCs, or hAMSC-CM for 24, 48, and 72 h. G CCK-8 assay for cell proliferation of LX-2 cells in different groups at different time points

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Stem Cell Research & Therapy

ISSN: 1757-6512