Fig. 6

DKK-3 and DKK-1 derived from hAMSCs inhibit LX-2 cell activation through blocking canonical Wnt signaling pathway. A Representative array images are shown (n = 4). DKK-3 and DKK-1 are highlighted with red boxes and arrows, accordingly. B Western blot detection of β-catenin, PPAR-γ, TGF-β, and α-SMA protein expression in LX-2 after ICG001 treatment. The normal LX-2 was used as control. C LX-2 cells were treated with normal medium (control), hAMSCs, and hAMSC-CM. The expression levels of GSK3β, P-GSK3β, β-catenin, and α-SMA in LX-2 cells of different groups were analyzed by western blot after 48 h of treatment. D LX-2 was cultured for 48 h in the presence of control siRNA or DKK-1/DKK-3 siRNA. Cell lysates were analyzed by western blot using antibodies against DKK-1 and DKK-3. E Quantitative analysis of the expression of DKK-1 and DKK-3 in hAMSCs cells of different groups as in (D). F Western blot analysis showed that the accumulation of β-catenin and the expression of α-SMA were increased in DKK-1 siRNA, DKK-3 siRNA, and DKK-1 + DKK-3 siRNA group when compared with control siRNA group