Fig. 7
RNA sequencing (RNA-seq) results. (A) Heat map of differentially expressed genes in ASCs, DFCs, and SHEDs after induction with pTDM under 3D conditions for 4 days in vitro. Significantly differentially expressed genes in these populations were determined based on RNA-seq results. Cut off: |log2 (foldchange) |> = 1 & Padj <  = 0.05. ASCs1, ASCs2, ASCs3, DFCs1, DFCs2, DFCs3, SHEDs1, SHEDs2, and SHEDs3 represent different biological replicates. (B) Pearson correlation analysis of biological samples, a correlation coefficient (R2) close to 1 indicates a higher similarity of expression patterns between samples. (C) Statistical histogram showed the number of differential genes in different combinations. Note: the number on the column indicated the number of differential genes. (D) The log2 (fpkm + 1) was used to generate a plot after centralized correction to obtain a gene list corresponding to each subset of the clustering line graph based on expression levels of differentially expressed genes in figure A. The abscissa indicated the sample name, and the ordinate indicated the expression value. The logarithm-centered corrected value was shown. The gray line in each subgraph represented the relative corrected gene expression levels of genes in a cluster among different experimental groups. The blue line represented the average relative corrected gene expression level of all genes in the cluster among different experimental groups. (E) Gene set enrichment analysis (GSEA) based on RNA-Seq data of ASCs/pTDM complex