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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Retinoic acid inhibits the angiogenesis of human embryonic stem cell-derived endothelial cells by activating FBP1-mediated gluconeogenesis

Fig. 4

Inhibition of FBP1 stimulated angiogenesis. a The qPCR analysis of FBP1 mRNA expression. b Representative western blot of FBP1. c Quantification of the FBP1 protein level in panel (b). d Representative bright-field images of EC spheroids. Scale bar, 100 μm. e Statistical analysis of the data in panel (d) for the total sprout length and sprout/spheroid ratio. f Representative bright-field images of wound healing. Scale bar, 100 μm. g Statistical analysis of the data in panel f for the scratch area and migration rate. h Cell cycle analysis of endothelial cells. i Statistical analysis of the data in panel (h). All data are presented as mean ± SEM; One-way ANOVA; *p < 0.05, **p < 0.01, and ns, not significant

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