Fig. 6

RA inhibits angiogenesis in vivo. a Representative images of Matrigel plug as highlighted in circle. The intensity of the red color and the level of transparency are parameters for qualitative evaluation. b Representative images of Matrigel plug paraffin section. Cells and Nuclei are stained with H&E. Matrigel is stained for pale background color. Vessel lumens are characterized by the absence of staining or filled with red blood and marked with asterisks. Scale bar, 100 μm. c Representative images of Matrigel plug paraffin section. Endothelial cells are stained for CD31 (green) and nuclei are stained with Hoechst 33,342 (blue). Scale bar, 50 μm