Fig. 1

hAFMSCs suppress pancreatic cancer cell proliferation. A Schematic for the in vitro hAFMSCs and PANC1 coculture system and functional assay. hAFMSCs and PANC1 cells were placed in the upper Transwell and lower well, respectively (Step 1). After 24 h of incubation, PANC1 cells were harvested from the wells and functional assays were performed, including proliferation assays (cell counting, cell cycle and colony-forming assays), migration assays (2-well culture inserts and Transwell assays) and invasion assays (Step 2). B Cell numbers were determined by a hemocytometer at 24 h and 55 h. C Representative images of colony formation of crystal violet-stained cells (left panel, scale bar = 5 mm) and quantitative statistics (right panel)