Fig. 2

hAFMSCs incude pancreatic cancer cell cycle arrest and apoptosis. A Cell cycle analysis of PANC1 cells alone or PANC1 cells cocultured with hAFMSCs for 24 h using flow cytometric analysis (left panel) and the percentages of each cell population at the different cell cycle phases were quantified (right panel). B Relative mRNA expression of cell cycle genes in PANC1 and Co-PANC1 cells. C (left) Western blot analysis of p21 in the cells and quantification is shown in the right panel. D Annexin V/propidium iodide (PI) staining of PANC1 cells alone or PANC1 cells cocultured with hAFMSCs for 48 h using flow cytometric analysis (left panel) and the percentages of apoptotic cell population were quantified (right panel). E Western blot analysis of pro-apoptotic molecules, including cleaved caspase3/8 and Bax in the cells and quantification is shown in the bottom panel. F Relative mRNA expression of pro-apoptotic genes in PANC1 and Co-PANC1 cells. Data are presented as the mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus the PANC1 group by Student’s t-test