Fig. 3

hAFMSCs suppress the migration ability of pancreatic cancer cells. A A wound healing assay was used to assess the gap closure rate of PANC1 cells cultured under different conditions at 40 h postincubation. Representative images of gaps at 0 h and 40 h are shown in the left panel and the right panel shows the percentages of cell-free areas at 40 h compared to those at 0 h determined by TScratch (scale bar = 100 μm). B Transwell migration assays were performed and the migrated PANC1 cells were stained with crystal violet and counted by ImageJ (scale bar = 1 mm). The quantified data are presented in the right panel. The results are shown as the mean ± SD of 3 independent experiments. ***P < 0.001 versus the PANC1 group by Student’s t-test