Fig. 6

hAFMSCs transplantation suppresses metastatic nodules in an orthotopic xenograft mouse model. A (left) Representative images demonstrating the whole-body dissection (ventral view) of nude mice receiving PBS orthotopic injection (control) and pancreatic cancer cells (PANC1). Green arrowhead indicates orthotopic pancreatic tumors. Scale bar = 1 cm. A (middle) Representative images of aligned intestines of control and PANC1 mice are shown and numerous nodules were observed on the mesentery of mice receiving PANC1 cell orthotopic injection. Red arrowhead indicates metastatic tumor nodules. Scale bar = 1 cm. A (right) Representative H&E staining images of metastatic nodules surround mesenteric lymph nodes are shown. Scale bar = 200 μm. Blue arrowhead indicates mesenteric lymph nodes and orange arrowhead indicates mesentery tissue. The magnified images were area within yellow dotted line and presented next to the H&E staining images. T: tumor cells. Scale bar = 50 μm. B Metastasis rate of nude mice under different treatments as follows: Control group, PANC1 implantations with PBS (PBS group) and with hAFMSCs intravenous injection (hAFMSCs group)