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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3β and NRF2

Fig. 2

Psoralen promoted bone regeneration of irradiated bone defects in a murine model. The image and quantitative data (BV/TV and BMD) of microCT showed that psoralen pretreatment enhanced the bone regenerative capacity of SSCs in irradiated bone defects (a and b). In addition, the results of HE, Masson, and Col-I staining demonstrated that Psoralen pretreatment of SSCs or irradiated SSCs yielded better bone formation than their counterparts without psoralen stimulation (ce). Moreover, psoralen pretreatment increased OCN-labeled OBs in the irradiated mice transplanted with SSCs or irradiated SSCs (f and g). All data are shown as the mean ± SD. **P < 0.01, *P < 0.05. The scale bars represent 2 mm (a), 500 μm (ce), and 200 μm (f), respectively. Control: irradiated bone defect; Vector: microgel; SSC: skeletal stem cell; IR: irradiation; Pso: psoralen; BV/TV: bone volume per tissue volume; BMD: bone mineral density; COL-I: collagen I; Ob.S/B.S: osteoblast surface per bone surface; and Ob.N/B.Pm: number of osteoblasts per bone perimeter;

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