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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3β and NRF2

Fig. 6

Blockade of PI3K/AKT and NRF2 partially abolished the promotion of psoralen-induced the proliferation and osteogenic activity of SSCs. As shown in a and b, blockage of AKT by LY294002 significantly abolished the psoralen-induced colony formation in SSCs and irradiated SSCs. The changes in the mRNA levels of the self-renewal-related genes Sox2 and Oct-4 further validated the CFU-F data (c and d). The ALP and von Kossa staining results showed that supplementation with ML385 (10 μM) partially abolished psoralen-induced osteogenesis in SSCs and irradiated SSCs (e and g). qPCR data showed that the expression of the osteogenic genes Sp7 and OCN was suppressed in the presence of ML385 (f ad h). All data are shown as the mean ± SD. **P < 0.01, *P < 0.05. The scale bars represent 5 mm (a) and 200 μm (e and g), respectively. SSC: skeletal stem cell; IR: irradiation; and Pso: psoralen

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