Fig. 4

Identification of hub genes by WGCNA for CRC stemness subtypes on GSE39582 dataset. a Scale independence and mean connectivity of multiple soft-thresholding powers (β) from 1 to 30. b The cluster dendrogram developed by the weighted correlation coefficients, genes with similar expression patterns were clustered into co-expression modules, and each color represents a module. c Heatmap of the correlation between module eigengenes (MEs) and clinical traits as well as stemness subtypes. d Scatter plot displaying relationship of module membership (MM) in turquoise module with gene significance for Cluster C3 (GS). e Top ten enriched biological process (BP), cellular component (CC) and molecular function (MF) GO terms of Cluster 3-associated module genes. f KEGG analysis of Cluster 3-associated module genes